Theoretical Biology & Bioinformatics

tree of life
Kirsten ten Tusscher

Kirsten ten Tusscher

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The research of the Ten Tusscher group focuses on the deciphering of developmental patterning processes in both plants and animals. For this the group uses state-of-the-art multi-scale modeling approaches and closely collaborates with experimental research labs. Our current research encompasses three major research lines:

  • Plant root development and adaptation to environmental conditions
  • Regulatory networks controlling cell fate decisions
  • Development and evolution of animal body axis segmentation

Plant root development

Unlike animals, plants keep growing and generating new organs throughout their life span, with formation of each new organ involving the de novo formation of a stem cell niche driving organ growth. Major research questions involve which processes pattern the stem cell niche and meristimatic region of dividing cells emanating from it, and which processes prepattern the locations along the main root competent for the future formation of lateral roots. In our research we use multi-scale cell-based models incorporating gene expression, hormonal signalling as well as growth, division expansion and differentiation of cells to answer these questions. Using this approach we previously demonstrated a division of labor between auxin and the downstream PLETHORA transcription factors in determining meristem size and rates of division, elongation and expansion (Mahonen et al., Nature, 2014). We subsequently recently demonstrated that the auxin-PLETHORA-ARR network controls activation, outgrowth and stabilisation of the root meristem after germination (Salvi et al, Dev Cell, 2020). Additionally, we have shown how lateral root priming emerges from the synergy between plant root tip auxin transport and root growth dynamics (van den Berg et al, BioRxiv, 2018).

Plant development also differs from animal development in that it is highly plastic, leading to non-stereotypical, environmentally dependent plant architectures. As an example, plant roots show directional growth, called tropisms, towards gravity but also away from salt. Additionally, in response to heterogeneous nutrient supplies, plant root systems show a preferential proliferation of roots in nutrient rich patches, called preferential root foraging. Tropisms can still be effectively studied by cell-based models of a single root, enabling us to succesfully identify additional genes contributing to the auxin asymmetry underlying root salt avoidance (van den Berg, Development,2016). However, to adress which processes give rise to an asymmetric growth of different parts of the root system in response to differences in their local nutrient conditions we need to expand to spatially more coarse-grainded FSP-type multi-scale models of overall root architecture. Using simplified models of this type we have already demonstrated that local, long-range and systemic nutrient signalling are likely insufficient to explain preferential nitrate foraging, and that the competition between roots for carbon resources further amplifies nutrient-difference induced asymmetries (Boer et al., 2020). To further enhance the realism and power of this overal root architecture modeling approach we recently developed our own biophysical model for water and carbon transport (van den Herik et al., Plant Cell Env, 2020) that can easily be integrated in this framework.

Cell fate decision making

During development cells have to decide whether to keep dividing and maintain an undifferentiated state, or rather differentiate and stop dividing. Additionally, upon differentiation choices between alternative cell fates have to be made. Importantly, in healthy non-cancer cells, the decision to differentiate is irreversible and terminal differentiation is mutually exclusive with an active division status. A key question is how the architecture and dynamics of the regulatory networks -genetic, epigenetic and posttranscriptional- controlling cell behavior give rise to these decisions in cell fate.

To adress these questions, we recently started a project in collaboration with the C.elegans groups of Sander van den Heuvel and Rik Korswagen, as well as with the labs of Alexander van Oudenaarden en Michiel Vermeulen. The overarching idea is to use single cell transcriptomics as well as epigenetic and protein data to reverse engineer the architecture and dynamic functioning of the network underlying mesoblast and neuron differentiation in C. elegans.

By combining omics based network inference and differential equation based modeling of gene expression dynamics, we aim to determine through sophisticated fitting and optimization procedures the architecture of the core regulatory networks involved.

Animal body axis segmentation

A segmented body axis occurs in three major animal clades, vertebrates, annelids and arthropods. In all three cases, the segments are prepatterned through an oscillatory processes occuring in the posterior part of the body. Through growth these oscillations are transformed into a spatially periodic stripe-like pattern, giving rise to an anterior-to-posterior sequence of formed segments.
plant model plant model
A major research question is whether this sequential segmentation mode was present in the urbilaterian ancestor and was subsequently lost in many animal clades or rather evolved independently in the clades. Additionally, it is unclear what the sequence of evolutionary innovations was that likely led to the innovation of segmented body plans.

To adress these questions, we use an in silico evo-devo approach, simulating the evolution of developmental processes in populations of simplified, multicellular developing organisms, each endowed with a genome encoding for a regulatory network controlling cell state. By replaying the in silico tape, varying mutation rates, selection pressures, and initial conditions, we investigate under which conditions segmentation evolves, the type of segmentation process evolving, and the order of evolutionary and mutational events.

Job opportunities

Currently there are no vacancies.

Group members

Current members

  • Kirsten ten Tusscher, Full professor, Principal Investigator
  • Thea van den Berg, PhD student, salt stress, lateral root priming
  • Jaap Rutten, PhD student, role of auxin cytokinin interactions in root growth
  • Bas van den Herik, PhD student, biophysics and regulation of sucrose transport
  • Milton Noguira da Silva Junior, PhD student, biophysics pf lateral root formation
  • Xiang Zhang, postdoctoral researcher, mechanics of root growth
  • Jerry Chen, PhD student, interplay plant physiology, ecology and climate
  • Alexandros Skourtis Cabrera, master student, interplay of PXY-CLE in determining secondary root growth
  • Jorian Flik, master student, deciphering the mechanism of root phototropims

Former members

  • Joana Teixeira Santos, PhD student, effect of shade avoidance on root architecture
  • Daniel Weisse, postdoctoral researcher, mechanics of root growth
  • Sophia Scheper, Master student, evolution of segmentation
  • Meine Boer, Master student, models of overall root system architecture
  • Thijs Geurts, Master student, directional responsesin roots, halotropism
  • Renske Vroomans, PhD student, evolution of segmentation in bilaterian animals
  • Peter de Greef, Master student, phosphate starvation
  • Bram van Dijk, Master student, Evolution of evolvability, genome versus network structuring
  • Rutger Bos, Master student, Pro's and cons of thermodynamic models for evolutionary simulations
  • Ioannis Tamvakis, Master student, Self-organised auxin patterning in plant development
  • Klaartje van Berkel, PhD student, Self-organization of auxin-PIN patterning in plant development
  • Jelmer de Ronde, Master student, Characterisation of the human ENCODE gene regulatory network
  • Redmar van den Berg, Master student, Polymorphism and speciation in diploid genome-network models
  • Janita Terpstra, Master student, Sympatric speciation in absence of assortative mating signals
  • Harold Wolff, Master student, Reconciling models for planar cell polarity